WebSep 9, 2013 · RNF185 transcripts increased in response to tunicamycin, peaking at 12 h after the onset of treatment (Fig. 4C, left panel), a time course comparable with the one observed for the bona fide UPR target GRP78 (Fig. 4C, right panel). WebDescription: Homo sapiens derlin 3 (DERL3), transcript variant 2, mRNA. RefSeq Summary (NM_001002862): The protein encoded by this gene belongs to the derlin family, and resides in the endoplasmic reticulum (ER). Proteins that are unfolded or misfolded in the ER must be refolded or degraded to maintain the homeostasis of the ER.
Anti-RNF185 antibody (ab73118) Abcam
WebOct 25, 2013 · To test the activity of RNF185 as a bona fide RING-dependent E3 ligase, we performed in vitro self-ubiquitination assays with GST-purified recombinant RNF185, in the presence of ubiquitin, ATP, E1, and different E2 enzymes. Auto-ubiquitination was monitored by Western blot using an anti-ubiquitin antibody (Fig. 2B).In the presence of UbcH5c, GST … WebNuclear envelope (NE) and endoplasmic reticulum (ER) collaborate to control a multitude of nuclear and cytoplasmic actions. In this context, the transmembrane protein TMEM147 localizes to both NE and ER, and through direct and indirect interactions hotpoint manuals download
Regulation of activated PAK-2p34 by proteasome mediated …
WebRNF185 is a E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. RNF185 acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent … Webxpression of RNF185-AS1 was analyzed by quantitative real-time PCR (qRT-PCR). Colony formation, 5-ethynyl-2′-deoxyuridine, and Cell Counting Kit-8 assays were utilized to determine cell proliferation. Cell migration and invasion were tested using wound healing and transwell assays. A mouse transplantation tumor model was used for tumor growth … WebSep 9, 2011 · Here we show that human RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy in cultured cells. The two C-terminal … line 8523 – meals and entertainment